Octet® R4 bio-layer interferometry system

Contact:

Prof. Janez Ilaš, M. Pharm., Ph. D.

Access:

The equipment is available at the Faculty of Pharmacy, Aškerčeva 7, 1000 Ljubljana. Use is possible upon agreement with the administrator. Due to the specificity of the equipment, the administrator must be present at all times when working with the instrument.

Intended Purpose:

Octet R4 (Sartorius) enables label-free real-time analysis of molecular interactions. The system enables 4-channel monitoring of interactions (binding affinity and kinetics) between molecules larger than 150 Da, as well as relative quantification of biological molecules (e.g. proteins, peptides, DNA, RNA, etc.) in complex samples. The work is carried out in 96-well microtiter plates (at least 180 μL of sample are required) at a temperature between 15 and 40 °C. Biosensors for various ways of ligand immobilization (e.g. covalent binding via amine groups, protein A for binding IgG, Ni-NTA for binding proteins harboring a hexahistidine tag (His-tag), etc.) are available.

Description of equipment:

Description of the equipment:

Octet® R4 instrument

Octet® BLI Discovery & Octet® Analysis Studio software

Price of equipment use: