Attune NxT Flow Cytometer with CytKick™ Max Autosampler

Contact:

Assoc. Prof. Anja Pišlar, M. Pharm., Ph. D.

Access:

Equipment is available at the University of Ljubljana, Faculty of Pharmacy, Aškerčeva 7, 1000 Ljubljana. Access to equipment must be agreed with supervisor of the equipment. Due to delicate nature of the equipment supervisor must be present through whole agreed working time on the equipment.

Intended Purpose:

Attune NxT flow cytometer with an automatic sample collection unit was developed with acoustic-assisted hydrodynamic focusing technology, and advanced fluidics designed to minimize clogging and effectively handle a broad range of cell types. This allows to run samples up to 10x faster and achieve greater resolution with little fear of sample loss due to clogging, for a higher degree of data, detail, and throughput that enables processing of a large range of sample types, including large clumpy cells, samples with a low concentration of cells, and precious samples, more quickly and accurately than ever before with no loss in data quality. Acoustic focusing technology enables sample flow rates up to 1,000 µL/min, without compromising data integrity. With the system, particles of size from 0.2 µm to 50 µm can be analysed with great reliability, precision and high resolution. The system with four lasers enables the analysis of 14 colour combinations, therefore the flow cytometer is suitable for the identification of molecular targets, the characterization of biochemical processes as well as the phenotyping of individual cell populations. The addition of the CytKick™ Max Autosampler enables significantly faster high-throughput screening on a multiparametric platform in different plate formats.

Description of equipment:

Attune NxT flow cytometer consists of a cell (pump) for sample injection, an optical system with "solid state flat top" diode lasers, electronics with an associated software package, and a part for storing working fluids and waste reagents. Attune NxT is a small device with dimensions of 40 cm x 58 cm x 43 cm, it is easy to use and maintain, and it is sufficient for normal laboratory conditions. The system is upgraded with an automatic sample collection unit CytKick Max Autosampler. This enables rapid processing of multiple samples by automatically analyzing samples from 96-well and 384-well plates (standard and deep-well plates), as well as sampling from custom-sized plates and 1.5 or 2 microcentrifuge tubes. It includes a "boost mode" that enables faster sample processing. In addition, by using special plate racks and a microcentrifuge, it enables passive cooling of samples.

Price of equipment use:

Use is xx € per hour.