HPLC Liquid chromatograph

Contact:

Assist. Zoran Lavrič, M. Pharm., Ph. D.

Access:

Equipment is available at the Faculty of Pharmacy, Aškerčeva 7, 1000 Ljubljana. Public access to the equipment is not forseen. In any case one has to agree the details of an eventual access with its supervisor which has to be present through whole agreed working time on the equipment.

Intended Purpose:

High pressure liquid chromatography system. Binary pump of the HPLC system is equipped with degasser and is capable of generating pressures up to 600 bar at a flowrate of up to 5 mL/min. Multisampler with temperature control of sample compartment has two drawers each holding 2 sample plates with 54 standard vials of 1.5 mL volume. Column heater module provides precise temperature control of column. Diode array detector with UV and VIS lamps has a variable slit size, records spectra with resolution of 1024 point, is capable of multichannel recording at different wavelengths as well as recording spectra for each time point to generate isoabsorbance maps. The system is used for various task in pharmaceutical analysis including dissolution testing, assay, solubility determinations and more generally to determine identity and quantity of analytes in complex heterogeneous samples that are produced at the Chair of Pharmaceutical Technology and serves as a tool that provides analytical feedback in the process of developing new dosage forms, formulations and processing technologies.

Description of equipment:

Price of equipment use